Proteomics - Methods and Instrumentation
Our key techniques are:
Electrophoresis
1D- and 2D-PAGE (e.g. vertical and horizontal carrier ampholyte IEF, IPG-IEF, SDS-PAGE with various buffer systems, large sized 2D-PAGE for simultaneously running up to twelve gels, all gel staining techniques)
Protein and peptide HPLC
RP-HPLC, IEX-HPLC, affinity chromatography (e.g. for depletion of high abundant proteins in serum or plasma), HPLC on monolytical columns, nano-HPLC separations All techniques are available both on analytical as well as preparative scale
Mass spectrometry
- ESI-MS: High resolution high mass accuracy mass spectrometry (LTQ-Orbitrap)
- MALDI-MS (4800 MALDI TOF/TOF Analyzer)
- Identification of PTMs
- Protein quantification via stable isotope labelling techniques (e.g. iTRAQ, ICPL, ICAT)
Bioinformatics
- Quantitative image analysis of 1D- and 2D-gels
- Mass spectra evaluation via e.g. Mascot, Sequest, ProteinPilot, de novo sequencing
- Biomarker discovery via multivariate statistics (e.g. PCA, PLS-DA)