Seibersdorf Academy

SIBCRA (Sampling and Identification of Biological, Chemical and Radiological Agents) Course - Group level

Course Aim

The scope of this course is to provide CBRN defense specialists with the required competence to analyze, plan, prepare for and collect chemical, biological, radiological and environmental samples under hazardous conditions. The scope also includes recording of the samples and maintenance of chain-of-custody.
The course does not include the collection of biomedical samples.


General Objectives

By the end of the training, trainees will be able to:

  1. Analyse the situation and identify the types of evidence available and samples to be collected by reviewing:
    • Observations and recordings of the reconnaissance team
    • Photographs
    • Video recordings
    • Witness statements
  2. Plan the collection of samples:
    • Define roles and responsibilities within the sampling team
    • Review procedures and work instructions
    • Identify the order of sample collection based on the presence of transient or fragile evidence and high value samples
    • Identify collection, packaging, labelling and recording requirements
    • Highlight the importance of maintaining chain-of-custody and the integrity of evidence
    • Identify the equipment required to implement the plan
    • Discuss safety aspects
  3. Prepare for the collection of samples:
    • Confirm the integrity of items in the sample collection kit
    • Prepare the sampling kit according to the sampling plan
    • Ensure all equipment is removed from packaging, ready for use
    • Clearly mark all sample containers with unique identifiers
    • Prepare documentation and chain-of-custody booklets
    • Obtain and prepare additional items required to implement the plan (eg. PPE, detectors, buckets and transports containers, etc.)
    • Ensure appropriate decontamination arrangements for samples are established
  4. Implement the sampling plan:
    • Approach the sampling locations in a safe manner
    • Assess the sampling locations to confirm the best sampling points and order of sample collection
    • Establish a clean area for the placement of the sampling kit
    • Work as part of the sampling team as a sub-team leader, sampling assistant and sampler
    • Safely collect soil, aqueous and wipe samples
    • Safely collect neat agent samples
    • Avoid cross contamination
      • Wear disposable gloves
      • Avoid direct contamination with contaminated surfaces
      • Limit contact with used and contaminated equipment to the sampler only
      • Collect one sample at a time
      • Individually seal samples
      • Change disposable gloves between samples and whenever contamination is suspected
      • Dispose of contaminated and used equipment (do not re-use)
    • Ensure accurate and timely recording of the sampling process in the documentation and chain-of-custody booklet
    • Deliver samples to the contamination control station
  5. Decontaminate and seal samples
    • Assess the order of decontamination and decontaminate similar sample types separately
    • Fully immerse samples in the decontamination solution for the required contact time
    • Ensure that samples are rinsed and checked for contamination
    • Ensure that sample containers are dried, secured with tape and sealed with frangible seals
    • Ensure that the samples are double bagged and sealed
    • Confirm that the seal numbers have been entered into the documentation and chain-of-custody booklet
  6. Maintain chain-of-custody
    • Sample in physical possession of a team member
    • Sample in constant view of a team member
    • Sample sealed and placed in a secure location


Course Content and Overview

The course will be held at the Seibersdorf Academy in Austria. Radiological emitters, industrial chemicals and biological simulants will be used. The course is divided into the following units, per week and material (RAD/BIO/CHEM):

Unit 1: Sampling Theory and Principles

  • Quality system framework (AEP 66)
  • Terms and definitions
  • Representative sampling
  • Sample prioritization
  • Sampling and analysis as part of evidence collection
  • Analysing information and intelligence
  • Preparing a Sampling Plan
  • Sampling team roles and responsibilities
  • Contamination control during sampling

Unit 2: Sampling kits and sample collection (individual skills)

  • Soil sample
  • Wipe sample
  • Aqueous sample
  • Neat agent collection and splitting
  • Air samples (optional)
  • Other samples

Unit 3: Sample collection (team skills)

  • Soil sample
  • Wipe sample
  • Aqueous sample
  • Neat agent collection and splitting

Unit 4: Collection of samples using simulants

  • Soil sample
  • Wipe sample
  • Neat agent collection

Unit 5: Collection of live agent samples

  • Soil sample
  • Wipe sample

Unit 6: Documentation and chain-of-custody

  • Principles of chain-of-custody
  • Documentation and chain-of-custody booklet

Unit 7: Decontamination

  • Sample decontamination
  • Sample sealing


Target Audience and maximum attendance

The course target audience are first responders and military personnel. The participants should be part of a response team. The maximum number of participants is 12.



  • CBRN advanced courses

Participants should be:

  • Medically and physically fit (Employer to provide a medical clearance for the employee to work in personal protective equipment)
  • Medical insurance valid in Europe/ Austria
  • Able to understand instructions/training delivered in the English Language
  • 18 years of age as a minimum
  • Female participants must not be pregnant


Method of Training

Delivery methods includes: Classroom and Practical Training



120 hours (minimum 16 days)
Timings and number of days are subject to customers' requirements and availability.

For more information please contact:

Seibersdorf Academy
Phone: +43 50550-3030

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