Proteomic

Chemical Analytics

Established workflows for proteomics projects

 

We offer a large variety of different workflows for proteomics projects, e.g.

 

Development of image analysis software for 1D- and 2D-gels

One of our latest projects was dedicated to the development of a 1D-image analysis software for the detection of doping with recombinant erythropoietin. The software was used at the IAAF World Championships in Athletics (Helsinki 2005) and the Olympic Winter Games (Torino 2006)

Quantitative proteomics for biomarker discovery

Digging deeper into proteomes by multidimensional fractionation techniques is one of the key elements for quantifying low abundant regulatory proteins. Our workflows include both gel based and non-gel based techniques and are selected according to the analytical needs and our customers‘ ideas. They are based on stable isotope labelling techniques on intact protein level or on peptide level after enzymatic digestion.
To mention just a few of our workflows: SDS-PAGE-MS, IPG-MS, 2D-PAGE-MS, multidimensional chromatography (mRP, SCX, RP, perfusion chromatography, multi affinity chromatography, etc.). Quantisation is usually done by HT-MALDI TOF/TOF mass spectrometry as well as latest ESI techniques (e.g. PQD, HCD) and dedicated bioinformatics software.

Characterization and identity confirmation of recombinant protein pharmaceuticals

The identity of generic protein/peptide based pharmaceuticals has to be strictly controlled. We compare these generic or non-generic pharmaceuticals with their non-generic analogues (e.g. erythropoietin, hCG, hGH, LH, FSH). A set of orthogonal techniques is usually employed (e.g. IEF-PAGE, 2D-PAGE, ESI-MSn, MALDI-TOF MS, HPLC on monolytic columns)

Proteomic profiling and classification

We use high resolution high mass accuracy mass spectrometry for this purpose. A series of several thousand characteristic masses per sample are being statistically evaluated and used for discrimination and grouping purposes.

 

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