Proteomics

Chemical Analytics

Proteomics - Methods and Instrumentation

Our key techniques are:

Electrophoresis

1D- and 2D-PAGE (e.g. vertical and horizontal carrier ampholyte IEF, IPG-IEF, SDS-PAGE with various buffer systems, large sized 2D-PAGE for simultaneously running up to twelve gels, all gel staining techniques)

Protein and peptide HPLC

RP-HPLC, IEX-HPLC, affinity chromatography (e.g. for depletion of high abundant proteins in serum or plasma), HPLC on monolytical columns, nano-HPLC separations All techniques are available both on analytical as well as preparative scale

Mass spectrometry

ESI-MS: High resolution high mass accuracy mass spectrometry (LTQ-Orbitrap)
MALDI-MS (4800 MALDI TOF/TOF Analyzer)
Identification of PTMs
Protein quantification via stable isotope labelling techniques (e.g. iTRAQ, ICPL, ICAT)

Bioinformatics

Quantitative image analysis of 1D- and 2D-gels
Mass spectra evaluation via e.g. Mascot, Sequest, ProteinPilot, de novo sequencing
Biomarker discovery via multivariate statistics (e.g. PCA, PLS-DA)

 

>> Proteomic workflows